Pringles Saline
1X in 1 Liter water

NaCl 9.0g
KCl 0.2g
CaCl 2 0.2g
dextrose
(glucose) 		4.0 g

 

Anticoagulant II (Mead et al.)
1X in 500 ml water

NaOH 1.96 g
NaCl
(or 10.7 g CaCl 2 )		 4.27 g

EDTA
(Na2 EDTA)

OR

3.16 g

EDTA(free acid)

2.48 g
Citric Acid (Na3Citric Acid.2H2O)
 6.03 g

OR one of these:

  

Andydrous Citric Acid

 3.94 g
Citric Acid.H2O
4.31 g

Adjust pH to 4.5 (pH is usually 4.1 after mixing, so slowly add NaOH)

do not use:
Na2 EDTA.H20
Na3 Citrate.2H20